Amplicon Sequencing

Amplicon sequencing is a targeted NGS method for analyzing genetic variation in specific genomic regions. It uses PCR to create DNA sequences called amplicons, enabling efficient variant identification and characterization. Multiplexing allows multiple samples to be sequenced together on a single run. Prior to multiplexing, individual samples must be transformed into libraries by adding adapters and enriching target regions through PCR. Adapters enable indexed amplicons to adhere to the sequencing flow cell. Amplicon sequencing can be used to discover rare somatic mutations in complex samples, among other applications.

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